ELISA Kit for Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1)
Ref: CC-SEE253Ra
48T, 96T, 96T×5, 96T×10, 96T×100
Información del producto
Especificidad:
This assay has high sensitivity and excellent specificity for detection of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1).
No significant cross-reactivity or interference between Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
13.7-10000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.2pg/mL
Research Area
Enzyme & Kinase
Organism species
Rattus norvegicus (Rat)
Alternative Names
Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I
Item Name
Prolyl-4-Hydroxylase Alpha Polypeptide I
Assay Length
3h
Method
Double-antibody Sandwich
Sample Type
tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Standards or samples are then added to the
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P54001