ELISA Kit for Endothelin Converting Enzyme 2 (ECE2)
Ref: CC-CEF414Hu
48T, 96T, 96T×5, 96T×10, 96T×100
Información del producto
Especificidad:
This assay has high sensitivity and excellent specificity for detection of Endothelin Converting Enzyme 2 (ECE2).
No significant cross-reactivity or interference between Endothelin Converting Enzyme 2 (ECE2) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endothelin Converting Enzyme 2 (ECE2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endothelin Converting Enzyme 2 (ECE2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
123.46-10000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 45.9pg/mL
Research Area
Enzyme & Kinase
Organism species
Homo sapiens (Human)
Alternative Names
Methyltransferase-like region
Item Name
Endothelin Converting Enzyme 2
Assay Length
2h
Method
Competitive Inhibition
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Endothelin Converting Enzyme 2 (ECE2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
O60344