Información del producto
CLIA Kit for Ethinylestradiol (EE)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Chemiluminescent immunoassay for Antigen Detection.
Detection Range
7.81-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 2.97pg/mL
Specificity
No significant cross-reactivity or interference between Ethinylestradiol (EE) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Ethinylestradiol (EE).
Organism species
Pan-species (General)
Item Name
Ethinylestradiol
Assay Length
2h
Method
Competitive Inhibition
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 100µ
7. Read RLU value immediately.
And then add 50µ
C
C
C
L Substrate Solution. Incubate 10 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 100µ
7. Read RLU value immediately.
And then add 50µ
C
C
C
L Substrate Solution. Incubate 10 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Ethinylestradiol (EE). A competitive inhibition reaction is launched between biotin labeled Ethinylestradiol (EE) and unlabeled Ethinylestradiol (EE) (Standards
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ethinylestradiol (EE) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ethinylestradiol (EE) were tested 20 times on one plate, respectively.
UniProt ID
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