Información del producto
Mini Samples ELISA Kit for Glutamic Acid (Glu)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
123.5-10,000ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 50.5ng/mL
Specificity
No significant cross-reactivity or interference between Mini Samples Glutamic Acid (Glu) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Glutamic Acid (Glu).
Organism species
Pan-species (General)
Item Name
Glutamic Acid
Assay Length
3h
Method
Competitive Inhibition
Sample Type
biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Glutamic Acid (Glu) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Glutamic Acid (Glu) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Glutamic Acid (Glu) were tested 20 times on one plate, respectively.
UniProt ID
-