Información del producto
Instant ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 5.5pg/mL
Specificity
No significant cross-reactivity or interference between Instant Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Instant Tumor Necrosis Factor Alpha (TNFa).
Research Area
Cytokine
Infection immunity
Tumor immunity
Infection immunity
Tumor immunity
Organism species
Rattus norvegicus (Rat)
Item Name
Tumor Necrosis Factor Alpha
Assay Length
1h, 10min
Method
Double-antibody Sandwich
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
Test Principle
10nm. The concentration of Instant Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the approp
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the approp
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively.
UniProt ID
P16599