Información del producto
CLIA Kit for Cathepsin K (CTSK)
Información adicional
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Chemiluminescent immunoassay for Antigen Detection.
Detection Range
5.49-4,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.94pg/mL
Specificity
No significant cross-reactivity or interference between Cathepsin K (CTSK) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Cathepsin K (CTSK).
Research Area
Bone metabolism
Enzyme & Kinase
Infection immunity
Enzyme & Kinase
Infection immunity
Organism species
Mus musculus (Mouse)
Item Name
Cathepsin K
Assay Length
2h, 40min
Method
Double-antibody Sandwich
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 100µ
8. Read RLU value immediately.
C
C
C
C
L Substrate Solution. Incubate 10 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 100µ
8. Read RLU value immediately.
C
C
C
C
L Substrate Solution. Incubate 10 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Cathepsin K (CTSK). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cathepsin K (CTSK). Next, Avidin
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested 20 times on one plate, respectively.
UniProt ID
P55097