Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
Ref: CC-MEA143Ra
96T
Información del producto
Mini Samples ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
15.6-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 6.0pg/mL
Specificity
No significant cross-reactivity or interference between Mini Samples Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Vascular Endothelial Growth Factor A (VEGFA).
Research Area
Apoptosis
Cytokine
Tumor immunity
Cytokine
Tumor immunity
Organism species
Rattus norvegicus (Rat)
Alternative Names
VEGF
Item Name
Vascular Endothelial Growth Factor A
Assay Length
3h
Method
Double-antibody Sandwich
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and add 25µ
4. Aspirate and wash 3 times
5. Add 25µ
6. Aspirate and wash 5 times
7. Add 25µ
8. Add 20µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 1 hour at 37°
2. Add 25µ
3. Aspirate and add 25µ
4. Aspirate and wash 3 times
5. Add 25µ
6. Aspirate and wash 5 times
7. Add 25µ
8. Add 20µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 1 hour at 37°
Test Principle
10nm. The concentration of Mini Samples Vascular Endothelial Growth Factor A (VEGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then adde
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then adde
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P16612