Información del producto
Instant ELISA Kit for Dehydroepiandrosterone (DHEA)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
123.5-10,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 50.1pg/mL
Specificity
No significant cross-reactivity or interference between Instant Dehydroepiandrosterone (DHEA) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Instant Dehydroepiandrosterone (DHEA).
Research Area
Endocrinology
Hormone metabolism
Reproductive science
Rheumatology
Hormone metabolism
Reproductive science
Rheumatology
Organism species
Pan-species (General)
Item Name
Dehydroepiandrosterone
Assay Length
1h, 10min
Method
Competitive Inhibition
Sample Type
serum, plasma and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 10 minutes at 37°
L standard or sample to each well.
Share and mix. Incubate 50 minutes at 37°
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 10 minutes at 37°
L standard or sample to each well.
Share and mix. Incubate 50 minutes at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Dehydroepiandrosterone (DHEA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Dehydroepiandrosterone (DHEA) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Dehydroepiandrosterone (DHEA) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
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