ELISA Kit for Pituitary Adenylate Cyclase Activating Peptide (PACAP)
Ref: CC-CEB347Mu
96T
Información del producto
ELISA Kit for Pituitary Adenylate Cyclase Activating Peptide (PACAP)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
24.69-2,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 9.19pg/mL
Specificity
No significant cross-reactivity or interference between Pituitary Adenylate Cyclase Activating Peptide (PACAP) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Pituitary Adenylate Cyclase Activating Peptide (PACAP).
Research Area
Endocrinology
Organism species
Mus musculus (Mouse)
Alternative Names
ADCYAP1
Item Name
Pituitary Adenylate Cyclase Activating Peptide
Assay Length
2h
Method
Competitive Inhibition
Sample Type
serum, plasma, tissue homogenates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 100µ
5. Aspirate and wash 5 times
6. Add 90µ
7. Add 50µ
And then add 50µ
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to ADCYAP1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ADCYAP1 and unlabeled ADCYAP1 (Standard
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pituitary Adenylate Cyclase Activating Peptide (PACAP) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
O70176