Información del producto
ELISA Kit for Heat Shock Protein 60 (Hsp60)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
1.56-100ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.65ng/mL
Specificity
No significant cross-reactivity or interference between Heat Shock Protein 60 (Hsp60) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 60 (Hsp60).
Research Area
Signal transduction
Tumor immunity
Tumor immunity
Organism species
Rattus norvegicus (Rat)
Alternative Names
HSPD1
Item Name
Heat Shock Protein 60
Assay Length
3h
Method
Double-antibody Sandwich
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 90µ
8. Add 50µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 3 times
5. Add 100µ
6. Aspirate and wash 5 times
7. Add 90µ
8. Add 50µ
C
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well. Incubate 2 hours at 37°
Test Principle
10nm. The concentration of Heat Shock Protein 60 (Hsp60) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 60 (Hsp60). Standards or samples are then added to the appropriate microti
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 60 (Hsp60). Standards or samples are then added to the appropriate microti
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 60 (Hsp60) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 60 (Hsp60) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P63039