Información del producto
ELISA Kit for Immunoglobulin G2 (IgG2)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
246.9-20,000ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 97.3ng/mL
Specificity
No significant cross-reactivity or interference between Immunoglobulin G2 (IgG2) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G2 (IgG2).
Research Area
Immune molecule
Infection immunity
Infection immunity
Organism species
Bos taurus
Bovine (Cattle)
Bovine (Cattle)
Alternative Names
IGHG2
Item Name
Immunoglobulin G2
Assay Length
2h
Method
Competitive Inhibition
Sample Type
serum, plasma and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 5 times
4. Add 90µ
5. Add 50µ
And then add 50µ
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 5 times
4. Add 90µ
5. Add 50µ
And then add 50µ
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A immediately.
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to Immunoglobulin G2 (IgG2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G2 (IgG2) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G2 (IgG2) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
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