Información del producto
Mini Samples ELISA Kit for Follicle Stimulating Hormone (FSH)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
2.47-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.88ng/mL
Specificity
No significant cross-reactivity or interference between Mini Samples Follicle Stimulating Hormone (FSH) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Mini Samples Follicle Stimulating Hormone (FSH).
Research Area
Endocrinology
Genetic science
Hormone metabolism
Reproductive science
Genetic science
Hormone metabolism
Reproductive science
Organism species
Mus musculus (Mouse)
Item Name
Follicle Stimulating Hormone
Assay Length
3h
Method
Competitive Inhibition
Sample Type
Serum, plasma and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
1. Prepare all reagents, samples and standards
2. Add 25µ
3. Aspirate and wash 3 times
4. Add 50µ
5. Aspirate and wash 5 times
6. Add 50µ
7. Add 25µ
And then add 25μL prepared Detection Reagent A immediately.
C
C
C
L Stop Solution. Read at 450 nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent B. Incubate 30 minutes at 37°
L standard or sample to each well.
Shake and mix. Incubate 1 hour at 37°
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FSH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FSH and unlabeled FSH (Standards or samples
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P01216