Información del producto
Instant ELISA Kit for Pepsin (PP)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antigen Detection.
Detection Range
2.47-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.94ng/mL
Specificity
No significant cross-reactivity or interference between Instant Pepsin (PP) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Instant Pepsin (PP).
Research Area
Enzyme & Kinase
Gastroenterology
Metabolic pathway
Tumor immunity
Gastroenterology
Metabolic pathway
Tumor immunity
Organism species
Homo sapiens (Human)
Item Name
Pepsin
Assay Length
1h, 10min
Method
Competitive Inhibition
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
2. Add 50µ
3. Aspirate and wash 3 times
4. Add 90µ
5. Add 50µ
C
C
L Detection Reagent A to each well. Incubate 1 hours at 37°
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L standard or sample and 50µ
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Instant Pepsin (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Instant Pepsin (P
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Pepsin (PP) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
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