ELISA Kit for Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1)
Ref: CC-AEA399Hu
96T
Información del producto
ELISA Kit for Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1)
Información adicional
Size
48T, 96T, 96T×5, 96T×10, 96T×100
Stabillity at RT
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the
Storage Conditions
-20°C
Shipping Conditions
Entre 4°C y 8°C
Applications
Enzyme-linked immunosorbent assay for Antibody Detection.
Detection Range
3.12-200ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 1.17ng/mL
Specificity
No significant cross-reactivity or interference between Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1).
Research Area
Infection immunity
Organism species
Homo sapiens (Human)
Alternative Names
HMG1
Item Name
High Mobility Group Protein 1
Assay Length
2h, 30min
Method
Competitive Inhibition
Sample Type
serum, plasma and other biological fluids
Format
48T, 96T, 96T×5, 96T×10, 96T×100
Assay Procedure
1. Prepare all reagents, samples and standards
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 5 times
5. Add 90µ
6. Add 50µ
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L standard or sample to each well. Incubate 2 hours at 37°
2. Add 100µ
3. Aspirate and add 100µ
4. Aspirate and wash 5 times
5. Add 90µ
6. Add 50µ
C
C
C
L Stop Solution. Read at 450nm immediately.
L Substrate Solution. Incubate 10-20 minutes at 37°
L prepared Detection Reagent A. Incubate 1 hour at 37°
L standard or sample to each well. Incubate 2 hours at 37°
Test Principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is
Precision
CV(%) = SD/meanX100
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1) were tested on 3 different plates, 8 replicates in each plate.
Intra-Assay: CV<10%
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-High Mobility Group Protein 1 Antibody (Anti-HMGB1) were tested 20 times on one plate, respectively.
Assay procedure summary
1. Prepare all reagents, samples and standards
UniProt ID
P09429