Recombinant Dengue Virus serotype 3 (Sri Lanka D3/H/IMTSSA-SRI/2000/1266) virus-like particles (VLP) consisting of Envelope, pre-Membrane and Membrane protein produced in HEK293 cells. The C terminal 20% of the Dengue Virus Envelope protein was replaced by the corresponding Japanese Encephalitis Virus SA-14 sequence (Envelope protein 399 a.a - 492 a.a). The replaced sequence corresponds to the transmembrane and intraparticle portion of the protein.