Información del producto
In addition to 5'-3' DNA polymerase activity, it also possesses 3'-5' exonuclease (proofreading) activity. Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, and is even up to ten-fold more accurate than normal Taq DNA polymerase. Consequently, Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.
Información adicional
Size
2500 Units
Storage Conditions
Store at -20C. Guaranteed stable for 6 months when properly stored.
Application Key
PCR
Supplied Product
10x Pfu buffer
10x Pfu Buffer with MgSO4
25 mM MgSO4
10x Pfu Buffer with MgSO4
25 mM MgSO4
Quality control testing
Free of detectable, non-specific nucleases.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.