Información del producto
T4 RNA Ligase is an ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly expressed by E.coli. This enzyme catalyzes the formation of phosphodiester bonds between oligonucleotides, single-stranded RNA and DNA intermolecular/intra-molecular 5'-PO4 and 3'-OH.
Información adicional
Size
500 uL
Storage Conditions
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
Application Key
IVT
Supplied Product
10x Reaction Buffer
ATP (10 mM)
PEG 8000
ATP (10 mM)
PEG 8000
Form
Liquid
Recomended Dilution
In Vitro Transcription
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer
50 mM KCl, 10 mM Tris-HCl, 1 mM DTT, 0.1mM EDTA, 50 % Glycerol, (pH 7.4)