Información del producto
mRNA Cap 2'-O-methyltransferase was derived from a recombinant E.coli strain that carries the gene for the vaccinia mRNA Cap 2'-O-Methyltransferase. This enzyme adds a methyl group at the 2'-O position of the first nucleotide adjacent to the cap structure at the 5' end of the RNA. The enzyme utilizes S-adenosylmethionine (SAM) as a methyl donor to methylate capped RNA (cap-0) resulting in a cap-1 structure. The Cap 1 structure can increase the translation efficiency, improving the expression of mRNA in transfection and microinjection experiments. This enzyme specifically requires RNA with an m7GpppN cap as substrate. It cannot utilize RNA with pN, ppN, pppN or GpppN at the 5'end. Capped RNA may be prepared via in vitro transcription using cap analog or through enzymatic capping using the Vaccinia Capping Enzyme.
Información adicional
Size
50 uL
Storage Conditions
Store at -20C or lower. Aliquot to avoid repeated freezing and thawing.
Application Key
IVT
Supplied Product
10x Capping Reaction Buffer
Form
Liquid
Recomended Dilution
In Vitro Transcription
The optimal working dilution should be determined by the end user.
The optimal working dilution should be determined by the end user.
Storage Buffer
20 mM Tris-HC (lpH 8.0), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol.