HotTaq PCR mix is a prepared solution containing everything needed for a successful PCR reaction except specific primers and DNA templates. The mix includes high quality recombinant Taq DNA polymerase, and nucleotides in a specifically optimized buffer formulation. For reaction set-up add the PCR Mix (25 ul) to the primers, template and water (is provided in the set; total volume of 50 ul). PCR Mix is provided with MgCl2 in the solution.