FluoTag-X4 anti-RFP two single-domain antibody (sdAb, also known as NanoAbTM) raised against the most common red fluorescent proteins.
Información adicional
Size200 uL
Storage ConditionsStore at -20C. For long term storage store at -80C. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Concentration5 uM
SpecificityRecognizes mRFP (red fluorescent protein) and other derivatives like mOrange, dsRed1, dsRED2, tdTomato, mCherry and mScarlet-i.
Application KeyIF
FormLyophilized
Recomended DilutionImmunofluorescence (1:500) The optimal working dilution should be determined by the end user.
Storage BufferLyophilized from 200 uL PBS containing 2.5 uM fluorescently labeled clone 1H1 and 1B2 (total concentration of fluorescently labeled sdAbs: 5 uM). Reconstitute with 200 uL 50% glycerol.
Clone Number2B12/2A1
ConjugationATTO 488 Two fluorophores of FluoTag-X4 series are site-specifically coupled to each individual FluoTag molecule.