FluoTag-X4 anti-GFP two single-domain antibody (sdAb, also known as NanoAbTM) raised against two distinct epitopes on GFP and its most common derivatives.
Información adicional
Size200 uL
Storage ConditionsStore at -20C. For long term storage store at -80C. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Concentration5 uM
SpecificityRecognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Application KeyIF
FormLyophilized
Recomended DilutionImmunofluorescence (1:500) The optimal working dilution should be determined by the end user.
Storage BufferLyophilized from PBS pH 7.4 containing 2.5 uM fluorescently labeled clone 1H1 and 2.5 uM fluorescently labeled clone 1B2. Reconsitute with 200 uL 50% glycerol.
Clone Number1H1/1B2
ConjugationAbberior STAR 635P Two fluorophores of FluoTag-X4 series are site-specifically coupled to each individual FluoTag molecule.