BRAF (phospho S446) polyclonal antibody
Ref: AB-PAB7907
BRAF (phospho S446) polyclonal antibody
Información del producto
Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF.
Información adicional
Size
100 uL
Gene Name
BRAF
Gene Alias
B-RAF1|BRAF1|FLJ95109|MGC126806|MGC138284|RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Storage Conditions
Store at -20C.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
Specificity
This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or
Application Key
WB-Ce,ELISA
Form
Liquid
Recomended Dilution
ELISA (1:2000)
Western Blot (1:1000) (Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.)
The optimal working dilution should be determined by the end user.
Western Blot (1:1000) (Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.)
The optimal working dilution should be determined by the end user.
Antigen species Target species
Human
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S446 of human BRAF. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).
Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide).
Gene ID
673