Información del producto
Rabbit polyclonal antibody raised against 6X His epitope tag peptide. The antibody is conjugated with Horseradish Peroxidase (HRP).
Información adicional
Size
100 ug
Storage Conditions
Store vial at 4C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
Specificity
This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this ant
Application Key
WB,IHC,ELISA
Immunogen Prot. Seq
HHHHHH
Form
Lyophilized
Recomended Dilution
ELISA (1:5000)
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2000)
Western Blot (1:2000-1:5000)
The optimal working dilution should be determined by the end user.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Iso type
IgG
Conjugation
HRP