Goat Anti-Mouse IgG F(c) secondary antibody (HRP)
Ref: AB-PAB31797
Goat Anti-Mouse IgG F(c) secondary antibody (HRP)
Información del producto
Goat polyclonal antibody raised against mouse IgG F(c) fragment. This secondary antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. The antibody is conjugated with Horseradish Peroxidase (HRP).
Información adicional
Size
20 mg
Storage Conditions
Store vial at 4C prior to restoration.
After reconstitution with 2.0 mL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
After reconstitution with 2.0 mL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
Specificity
IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against mouse IgG F(ab).
Application Key
WB,IHC,ELISA
Form
Lyophilized
Recomended Dilution
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Antigen species Target species
Mouse
Immunogen
Mouse IgG F(c) fragment.
Storage Buffer
Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Conjugation
HRP