Rabbit Fab Anti-Human IgG (H&L) secondary antibody (HRP)
Ref: AB-PAB31783
Rabbit Fab Anti-Human IgG (H&L) secondary antibody (HRP)
Información del producto
Rabbit Fab polyclonal antibody raised against human IgG (H&L). The antibody is conjugated with Horseradish Peroxidase (HRP).
Información adicional
Size
500 ug
Storage Conditions
Store vial at 4C prior to restoration.
After reconstitution with 500 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
After reconstitution with 500 uL deionized water (or equivalent), aliquot contents and freeze at -20C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not
Specificity
IgG (H&L). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-rabbit serum. No reaction was observed against anti-papain or anti-rabbit IgG F(c).
Application Key
WB,IHC,ELISA
Form
Lyophilized
Recomended Dilution
ELISA (1:10000-1:50000)
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Immunohistochemistry (1:500-1:2500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
Antigen species Target species
Human
Immunogen
Human IgG whole molecule.
Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (immunoglobulin and protease free), 0.01% (w/v) gentamicin sulfate). Do NOT add sodium azide!
Iso type
IgG Fab
Conjugation
HRP