Storage ConditionsStore at -20C. Aliquot to avoid repeated freezing and thawing.
SpecificityThis antibody detects endogenous levels of Smad3 protein only when phosphorylated at S204.
Application KeyWB-Ce,IHC-P,IF,ELISA
FormLiquid
Recomended DilutionELISA (1:20000) Immunohistochemistry (1:100-300) Immunofluorescence (1:200-1000) Western Blot (1:500-2000) The optimal working dilution should be determined by the end user.
Antigen species Target speciesHuman
ImmunogenA synthetic peptide corresponding to amino acids 140-220 of human SMAD3 (phospho S204).