Storage ConditionsStore at -20C. Aliquot to avoid repeated freezing and thawing.
SpecificityAKT1/AKT3 (phospho Y437/Y434) polyclonal antibody detects endogenous levels of human AKT1/AKT3 only when phosphorylated at tyrosine 437 and tyrosine 434.
Application KeyWB-Ce,IHC-P
FormLiquid
Recomended DilutionImmunohistochemistry (1:50-1:100) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
Antigen species Target speciesHuman
ImmunogenSynthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y437/Y434 of human AKT1/AKT3.
Storage BufferIn PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)