Storage ConditionsStore at -20C. Aliquot to avoid repeated freezing and thawing.
SpecificitySRF (phospho S77) polyclonal antibody detects endogenous levels of human SRF only when phosphorylated at serine 77.
Application KeyWB-Ce,IHC-P
FormLiquid
Recomended DilutionImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
Antigen species Target speciesHuman
ImmunogenA synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S77 of human SRF.
Storage BufferIn PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)