Storage ConditionsStore at 4C. For long term storage store at -20C. Aliquot to avoid repeated freezing and thawing.
SpecificityThis affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pT179 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the
Application KeyWB-Ce,IHC,ELISA
FormLiquid
Recomended DilutionELISA (1:15000-1:75000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Antigen species Target speciesHuman
ImmunogenSynthetic phosphopeptide corresponding to residues surrounding T179 of human SMAD3.
Storage BufferIn 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)