Información del producto
Phospho PKC θ (Y90) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
IHC
WB
WB
Concentration
1 mg/ml
Recomended Dilution
IHC-p 1:50-300
WB 1:500-2000
WB 1:500-2000
Type Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human Phospho PKC θ (Y90)
Subcellular Location
Cytoplasm. Cell membrane
Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
5588
Observed Band
78kD
Other Name
Protein kinase C theta type (EC 2.7.11.13
nPKC-theta)
nPKC-theta)
Background
Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme