Storage Conditions
-20°C/1 year
Applications
ELISA
WB
Concentration
1 mg/ml
Recomended Dilution
WB 1:500-2000 ELISA 1:5000-20000
Type Clonality
Polyclonal
Immunogen
Synthesized peptide derived from human protein . at AA range: 30-110
Subcellular Location
Nucleus.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
2958
Observed Band
11kD
Background
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A
MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit
MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF
MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respec