Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF7. AA range:311-360
Subcellular Location
Cell junction, focal adhesion . Cell projection, ruffle . Cytoplasm, cell cortex . Cell projection, lamellipodium . Detected at cell adhesions. A small proportion is detected at focal adhesions.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
8874
Observed Band
80kD
Other Name
ARHGEF7
Beta-Pix
COOL-1
COOL1
KIAA0142
Nbla10314
P85SPR
PAK-interacting exchange factor beta
PAK3BP
PIXB
Rho guanine nucleotide exchange factor 7
p85
Background
This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffle