Información del producto
PKC θ (phospho Thr538) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553
Subcellular Location
Cytoplasm. Cell membrane
Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
5588
Observed Band
81kD
Other Name
PRKCQ
PRKCT
Protein kinase C theta type
nPKC-theta
PRKCT
Protein kinase C theta type
nPKC-theta
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved