Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human n-NOS around the phosphorylation site of Ser852. AA range:818-867
Subcellular Location
Cell membrane, sarcolemma
Peripheral membrane protein. Cell projection, dendritic spine . In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By si
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Monkey
Mouse
Rat
Human Gen
4842
Observed Band
130-160kD
Other Name
Constitutive NOS
N-NOS
NC-NOS
NOS1
NOS type I
Neuronal NOS
Nitric oxide synthase
Peptidyl-cysteine S-nitrosylase NOS1
bNOS
brain
nNOS
Background
The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission,