Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573
Subcellular Location
Cell membrane
Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By
Cytoplasmic side . Cell projection, microvillus membrane
Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane
Peripheral membrane protein
Peripheral membrane protein
Peripheral membrane protein
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
4478/5962
Observed Band
67kD
Other Name
Cytovillin
EZR
Ezrin
MSN
Membrane-organizing extension spike protein
Moesin
RDX
Radixin
VIL2
Villin-2
p81
Background
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopo