Información del producto
Smad2 (phospho Thr220) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
WB 1:500-2000,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000,WB 1:500-2000
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad2 around the phosphorylation site of Thr220. AA range:186-235
Subcellular Location
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
4087
Other Name
JV18-1
MADH2
MADR2
MAD homolog 2
Mad-related protein 2
Mothers against DPP homolog 2
Mothers against decapentaplegic homolog 2
SMAD 2
SMAD2
SMAD family member 2
Smad2
hMAD-2
hSMAD2
MADH2
MADR2
MAD homolog 2
Mad-related protein 2
Mothers against DPP homolog 2
Mothers against decapentaplegic homolog 2
SMAD 2
SMAD2
SMAD family member 2
Smad2
hMAD-2
hSMAD2
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional mo