Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human TF2A1. AA range:281-330
Subcellular Location
Nucleus.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
2957
Observed Band
42kD
Other Name
GTF2A1
General transcription factor IIA subunit 1
TF2A1
TFIIA-42
TFIIAL
Transcription initiation factor IIA subunit 1
Transcription initiation factor TFIIA 42 kDa subunit
Background
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A
MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010],