Storage Conditions
-20°C/1 year
Applications
ELISA
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human GRF-1 (phospho Tyr1087)
Subcellular Location
Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm . Nucleus . Cell membrane . In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymeriz
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
2909
Observed Band
190kD
Other Name
ARHGAP35
GRF-1
GRF1
GRLF1
Glucocorticoid receptor DNA-binding factor 1
Glucocorticoid receptor repression factor 1
KIAA1722
Rho GAP p190A
Rho GTPase-activating protein 35
p190-A
Background
The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequence