Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human SIAH1. AA range:181-230
Subcellular Location
Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
6477/6478
Observed Band
34kD
Other Name
E3 ubiquitin-protein ligase SIAH1
E3 ubiquitin-protein ligase SIAH2
HUMSIAH
SIAH1
SIAH2
Seven in absentia homolog 1
Seven in absentia homolog 2
Siah-1
Siah-1a
Siah-2
hSiah2
Background
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has