Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunocytochemistry: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human ROCK2. AA range:691-740
Subcellular Location
Cytoplasm. Cell membrane
Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Monkey
Mouse
Rat
Human Gen
9475
Observed Band
165kD
Other Name
KIAA0619
ROCK-II
ROCK2
Rho-associated
Rho-associated, coiled-coil-containing protein kinase II
Rho-associated protein kinase 2
Rho kinase 2
coiled-coil-containing protein kinase 2
p164 ROCK-2
Background
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is a