Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human B-RAF. AA range:576-625
Subcellular Location
Nucleus . Cytoplasm . Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
673
Observed Band
85kD
Other Name
BRAF
BRAF1
Proto-oncogene B-Raf
RAFB1
Serine/threonine-protein kinase B-raf
p94
v-Raf murine sarcoma viral oncogene homolog B1
Background
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this