Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230
Subcellular Location
Nucleus .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Monkey
Mouse
Rat
Human Gen
6688
Observed Band
32kD
Other Name
31 kDa-transforming protein
SPI1
Transcription factor PU.1
Background
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regul