Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human MIF. AA range:25-74
Subcellular Location
Secreted . Cytoplasm . Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
4282
Observed Band
12kD
Other Name
GIF
GLIF
Glycosylation-inhibiting factor
L-dopachrome isomerase
L-dopachrome tautomerase
MIF
MIF
MMIF
Macrophage migration inhibitory factor
Phenylpyruvate tautomerase
Background
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This l