Información del producto
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from in-ternal human MDM2. AA range:132-181
Subcellular Location
Nucleus, nucleoplasm. Cytoplasm . Nucleus, nucleolus. Nucleus . Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MD
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Monkey
Mouse
Monkey
Mouse
Human Gen
4193
Observed Band
90kD
Other Name
Double minute 2 protein
E3 ubiquitin-protein ligase Mdm2
Hdm2
MDM2
Oncoprotein Mdm2
p53-binding protein Mdm2
E3 ubiquitin-protein ligase Mdm2
Hdm2
MDM2
Oncoprotein Mdm2
p53-binding protein Mdm2
Background
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpres