Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H1. AA range:1-50
Subcellular Location
Nucleus. Chromosome. According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Human Gen
3009/3007/3008
Observed Band
31 28kD
Other Name
H1F3
H1F4
H1F5
HIST1H1B
HIST1H1D
HIST1H1E
Histone H1.3
Histone H1.4
Histone H1.5
Histone H1a
Histone H1b
Histone H1b
Histone H1c
Histone H1s-2
Histone H1s-3
Histone H1s-4
Background
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in