Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
IP
WB
Concentration
1 mg/ml
Recomended Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000.IP 1:200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
Subcellular Location
Cytoplasm . Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602). .
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
3091
Observed Band
92-130kD
Other Name
ARNT-interacting protein
BHLHE78
Basic-helix-loop-helix-PAS protein MOP1
Class E basic helix-loop-helix protein 78
HIF-1-alpha
HIF1-alpha
HIF1A
Hypoxia-inducible factor 1-alpha
MOP1
Member of PAS protein 1
PASD8
PAS doma
bHLHe78
Background
hypoxia inducible factor 1 alpha subunit(HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master reg