Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110
Subcellular Location
Cytoplasmic side.
Cytoplasmic side. Microsome membrane
Single-pass membrane protein
Single-pass membrane protein
[Isoform 1]: Endoplasmic reticulum membrane
[Isoform 2]: Cytoplasm.
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Human Gen
1528
Observed Band
15kD
Other Name
CYB5
CYB5A
Cytochrome b5
MCB5
Microsomal cytochrome b5 type A
Background
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglob