Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220
Subcellular Location
Endoplasmic reticulum membrane
Multi-pass membrane protein .
Multi-pass membrane protein . Golgi apparatus membrane
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Human Gen
55331
Observed Band
35kD
Other Name
ACER3
APHC
AlkCDase 3
Alkaline CDase 3
Alkaline ceramidase 3
Alkaline dihydroceramidase SB89
Alkaline phytoceramidase
PHCA
aPHC
Background
enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiqu