Información del producto
NFκB-p65 (phospho Ser311) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser311. AA range:278-327
Subcellular Location
Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleu
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Mouse
Human Gen
5970
Observed Band
65kD
Other Name
NFKB3
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
RELA
Transcription factor p65
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
RELA
Transcription factor p65
Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcripti