Información del producto
Smad3 (phospho Ser208) rabbit pAb
Información adicional
Storage Conditions
-20°C/1 year
Applications
ELISA
IF
IHC
WB
IF
IHC
WB
Concentration
1 mg/ml
Recomended Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Type Clonality
Polyclonal
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser208. AA range:174-223
Subcellular Location
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/S
Iso type
IgG
Tissue Source
Rabbit
Reactivity
Human
Mouse
Rat
Mouse
Rat
Human Gen
4088
Observed Band
40kD
Other Name
JV15-2
MADH3
MAD homolog 3
Mad3
Mothers against DPP homolog 3
Mothers against decapentaplegic homolog 3
SMAD3
SMAD 3
SMAD family member 3
Smad3
hMAD-3
hSMAD3
MADH3
MAD homolog 3
Mad3
Mothers against DPP homolog 3
Mothers against decapentaplegic homolog 3
SMAD3
SMAD 3
SMAD family member 3
Smad3
hMAD-3
hSMAD3
Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional mo